'29121'

'24'

'普洱熟茶中8氧化物咖啡因和嘧啶生物碱的存在'

'/puerchazhishi/a-29121.html'

''

''

'晒青毛茶,双江勐库,临沧市,普洱茶,生茶,熟茶,勐库,临沧,绿茶,普洱,嘧啶,咖啡因,生物碱,层析,化合物,氧化,儿茶素,中分,吡喃糖,双江'

'普洱茶是以云南大叶茶[ Camellia sinensis(Linn .) var. assamica (Masters ) Kitamura ] 为原料制作的茶类。普洱茶可分为普洱生茶(Pu-Er raw tea) , 普洱陈茶( Pu-Er aging tea ) 和普洱熟茶(Pu-Er ripe tea) 等三...'

'0'

'1'

'中华普洱茶网编辑'

'中华普洱茶网'

'9'

'0'

'0'

'CAIJI'

'2018-05-29 14:03:01'

'2018-05-29 14:03:01'

'0'

''

'0'

'<p>普洱茶是由云南大叶茶 (assamica) (次郎) 为原料制成的。普洱茶可分为三系列 (pu 二、2006)、普洱茶 (老普洱茶) 和普洱熟茶 (熟、普洱)、普洱茶。普洱茶不是由绿茶发酵而成, 可作为绿茶饮用, 并通过发酵过程进入普洱茶或普洱煮熟的茶叶中。普洱茶是由普洱茶发酵过程中的微生物参与而产生的。</p><br><p>我们研究了普洱茶的化学成分, 从普洱茶中分离出大量的酚类化合物, 包括: (+) 儿茶素、(-) 儿茶素 (EC)、无食子儿茶素 (GC)、(-) 表无食子儿茶素 (胃癌)、(-) 表儿茶素-3 o-未食子酸酯 (心电图), (-) 表无儿茶素-3-邻酸酯 (EGCG), (-) 表富--3-邻没食子酸酯, 槲皮素, 槲皮素-3 o β d-葡萄吡咯苷, 芦丁, Shannai 苯酚, Shannai 苯酚-3 o β d-葡萄, Shannai phenol-3 o 芦丁, 小木麻黄 (Saictinin), L, 6-两个无食子酰酚酞葡萄糖, 茶倍性, 绿原酸, 3α, 5α-两羟基-4 α邻咖啡酰基金鸡酸, 松醇苷, 未食子酸;和咖啡因 (周郑智洪和杨崇仁, 2000)。</p><br><p>普洱茶的化学成分与普洱茶有显著的差异, 我们报告了 Nasso (Cinchonain) 酚类化合物, 包括普洱茶 (Puerin) a (1) 和 B (2), 以及儿茶-[7, 8 BC]-4α-(4-h ydroxyl-苯基)-dihydro-2 (3 小时)-pyranone (3) 和 Cinchonain Ib (4), 普洱成熟茶的特征成分 (图 1)。其中, 化合物1和2是新化合物, 3 ~ 4 最初与茶树分离。从普洱茶我们也分离了 (+)-儿茶素 (C), (-) 表儿茶素 (EC), 没有食物儿茶素 (GC), (-) 表无儿茶素 (胃癌), (-) 表未食子酸葡糖苷, 未食子酸, Shannai 苯酚, Shannai 苯酚-3 o β d-葡萄-口服糖苷,Shannai 苯酚,-3 o 芦丁, 槲皮素, 槲皮素-3 邻β d, 杨梅素 (杨梅素), 2,-2′-, 6, 6′-第四纪苯和 2, 5-二羟基苯甲酸, 和其他酚类化合物, 以及咖啡因 (周 et, 2005;林芷等, 200 6)。</p><br><p>为了进一步阐明普洱的物质基础, 系统的化学研究仍在进行中。本文根据现有的工作, 对云南省双江生产的普洱茶中的生物碱和酚类化合物进行了报道。</p><br><br><p>普洱煮茶配料</p><br><br><br><p>图1普洱茶的特征成分</p><br><br><p>1实验部分</p><br><p>1. 1 仪器和材料</p><br><p>1 d, 2 d 核磁共振波谱在氘替换的溶剂 (CD3 COCD3 + d2o,cd3od) 由布鲁克 optics AM-400 和 DRX-500 核磁共振, TMS 为内部标准, 化学位移δ在 ppm (10-6), 耦合常数 J 与 Hz。用 VG 自动 Spec-3000 质谱仪对质谱进行了测量。柱层析与 Diaion HP20SS (三菱化学公司), Mci 凝胶 CHP-20 (75 ~ 150μm, 三菱化学公司),</p><br><p>葡 LH-20 (25 ~ 100μm, 法玛西亚精细化工有限公司), Chromatorex ODS (~ 网, 富士 Silysia 化工有限公司) 和硅胶 H (青岛海洋化工厂), 薄层层析与预制硅胶板 H (0.20 ~ 0 毫米, 青岛海洋化工厂, 薄层色谱膨胀剂为苯乙酸乙酯-甲酸或氯仿-甲醇-水, 显色剂为5% 氯化铁乙醇溶液或5% 硫酸乙醇溶液, 加热颜色。</p><br><p>普洱熟茶样本来自云南省临沧市双江县双江孟库茶厂生产。</p><br><p>1. 2 提取和分离</p><br><p>普洱茶 (千克) 与60% 丙酮在室温冷浸3次, 过滤, 合并滤液, 减压和回收丙酮通过葡 LH-20 柱层析, Meoh-h2 O (1:0 ~ 0:1) 和50% 丙酮梯度洗脱, 11 组分 (法郎 1-11)。1是反复分离的 Mci-凝胶 Chp-20p,sephadex LH-20 和 Chromatorex ODS 柱层析, MEOH-H2O 系统洗脱, 化合物 5 (7MG), 6 (毫克), 7 (毫克) 和 8 (5 m g)。2. 化合物 9 (2 毫克) 和 (15mg) 通过 Meohh2o 梯度洗脱与 Mci 凝胶 chp-20p 和 SephadexLH-20, Chromatorex ODS 柱层析纯化。8, 用 Mci 凝胶 chp-20p, Toyopearl hw-40f 和 SephadexLH-20 柱层析重复分离, Meoh-h2o 洗脱, 获得 (7MG)。再次, 从10的成分得到了从化合物 (MG)。</p><br><p>1. 3 鉴定</p><br><p>胞嘧啶核苷 (胸苷) (5), 白色粉末, 晶圆-ms m/z 241 [m h]-;1 H 核磁共振 (Mhz,cdcl3) δ8。(s, H-6), 1。(s, H-7), 6。(T, J = 6.9, 6.7 hz,1′-H), 2。(M,-2′-), 4。(m, 3′), 4 (m, 4′-h), 4。(dd, J = 8, 3 Hz, 5′ H) 和 C 先生 (100MHz, CDCl3) δ12。1 (C-7), 9 (c-2), 2 (c-3), 85。8 (c-1), 87。5 (c-4), 111。0 (C-5), 137。5 (C-6), 151。5 (C-2), 165。5 (C-4)。(三郑等, 1995)</p><br><p>胸腺嘧啶 (嘧啶) (6), 白色粉末, Ei-ms m/z 126; 1 h 核磁共振 (400MHz, CDCl3) δ8。(s, H-6), 1。CDCl3 (S, H-7) 和 C 核磁共振 (100MHz,) δ153.6 (C-2), 166。6 (C-4), 109 0 (C-5), 138。9 (C-6), 5 (C-7)。</p><br><p>尿嘧啶 (尿) (7), 白色粉末, 负离子, 晶圆 +-ms m/z 113 [m + H] +;1 H 核磁共振 (400MHz, CDCl3) δ5。(d, J = 7.6 Hz, H-5), 7。(d, J = 7.6 Hz, H-6) 和 C 核磁共振 (100MHz, CDCl3) δ151 5 (C-2), 164。4 (C-4), 110。0 (C-5), 2 (C-6)。</p><br><p>8-咖啡因氧化物 (8-oxocaffeine) (8), 白色粉末, 负离子-ms m/z 209 [m h]-;1 H 核磁共振 (400MHz, CD3 Cl3) δ3。(s, NMe), 3。(s, NMe), 3 (s, NMe)。CNMR (兆赫, CDCl3) δ28。2 (我), 28。6 (i), 2 (i), 100。1 (C-4), 148。3 (C-5), 151。5 (C-2), 154。7</p><br><p>(C-6), 162。0 (C-8)。(Stadler 和菲, 1995)</p><br><p>黄七糖苷 (engeletin) (9), 黄粉, 负离子, 晶圆-ms m/z 431 [m h]-;1H 核磁共振 (兆赫, Dmso-d6), δ6。(Br S, H-6), 6。(Br S, H-8), 6 (d, J = 8 6Hz, h-2), 7。(d, j = 8.6 hz, h-3), 7 (d, j = 8.6 Hz, h-5), 6。(d, j = 8 6Hz, h-6), 5 (d, rhah-6), 5 (d, RhaH-1), 1 (d, j = 6 0 Hz, RHA H-6)。(Krishnamachari 等, 2002)</p><br><p>1, 2, 4-对苯二酚 (1, 2, 4-benzenetriol) (10), 白色粉末, 负离子-ms m/z [m h]-;1 h-核磁共振 (400MHz, cd3od): Δ6。(H, Br S, H-3), 7。已修改 (1H、d、j = 8 2 hz、H-4)、6 (1H、D、j = 8.2 Hz、H-6)。</p><br><p>1, 3-对苯二酚 (1, 3-酚) (11), 白色粉末, 负离子-ms m/z 109 [m h]-;1H 核磁共振 (mhz,cd3od), δ6。(2H, D, J = 8.9 Hz, H-2, 4), 8。(1 小时, d, J = 8.9 Hz, H-3), 7。(1H, Br, H-6)。</p><br><p>4-甲基-1, 2-二苯酚 (4-甲基-1, 2-酚) (12), 白色粉末, 负离子-ms m/z 124 [m h]-;1 h-核磁共振 (400MHz, cd3od): Δ7。(1H, BRS, H-3), 6。(1H, d, j = 8.2 hz, H-5), 7 (1H, D, j = 8.2 Hz, H-6);CNMR (100MHz, Cd3od): δ149 6 (S, C-1), δ144。5 (S, C-2), 5 (d, C-3), 123。9 (S, C-4), 123。4 (d, C-5), 117。2 (d, C-6), 50。2 (q, C-7)。</p><br><br><p>2结果和讨论</p><br><p>云南省双江生产的普洱茶中丙酮提取物由葡 LH-20、Mci 凝胶 chp-20p、Chromatorexods 和 Toyopearl hw-40f 分离, 获得8种化合物, 包括8氧化物咖啡因 (8-oXocaffeine) (8) (Stadler 和菲, 1995) 和嘧啶生物碱: 胸苷 (胸苷) (5), 胸腺嘧啶 (嘧啶) (6) 和氟尿嘧啶 (尿) (7), 含糖黄酮类: 黄七苷 (engeletin) (9) (克瑞史 namachari, 2002), 以及简单酚类化合物: 1, 2, 4-对苯二酚 (1, 2, 4-benzenetriol) (10), 1, 3-氢醌 (1, 3-酚) (11) 和 4-甲基-1, 2-二苯酚 (4-甲基-1, 2-酚) (12) (图 2)。这些化合物首先与普洱茶分离。</p><br><br><p>复合 5 ~ 12</p><br><br><p>图2云南省双江生产的普洱熟茶中分离出复合 5 ~ 12</p><br><br><p>已知茶叶富含嘌呤类生物碱化合物, 如: 咖啡因、可可碱、茶碱、黄嘌呤、乌头碱等。据报道, 8 氧化物咖啡因 (8) 是由咖啡因在油炸, 研磨和瞬时加工过程中的氧化形成的, 这是加工咖啡的象征成分, 具有显著的抗氧化活性, 并没有被分离茶。我们先从普洱茶中分离出来的 8-咖啡因 (8), 很明显, 普洱茶在高温和潮湿的过程中发酵后, 在微生物的作用下, 由生物转化的产品, 为普洱茶的质量和生理活动评价具有重要的意义。</p><br><p>嘧啶生物碱 (如: 氟尿嘧啶、胸腺和胞嘧啶) 也存在于诸如茶叶等高等植物中。核苷和核苷是微生物和动物生殖细胞的组成部分, 在植物中很少发现。这种化合物参与 dna 代谢的过程, 由 dna 和 RNA 组成, 具有显著的生理活性 (Kinahan ET, 1981)。核苷 Smilacina (5) 主要见于海洋生物, 也据报道与贝母、红叶小檗分离 (三郑、1995;Razhigang, 1997;Chonghong, 1999;Yangshunli 和刘戏曲, 2003), 是一个中间体的合成抗艾滋病药物司他夫定和齐多夫定 (AZT)。首次从成熟茶叶中分离出了胸腺嘧啶 (5) 的普洱茶, 可能是由于嘧啶生物碱发酵过程中的凝结形成了茶叶中的核苷化合物。</p>'

'晒青毛茶,双江勐库,临沧市,普洱茶,生茶,熟茶,勐库,临沧,绿茶,普洱,嘧啶,咖啡因,生物碱,层析,化合物,氧化,儿茶素,中分,吡喃糖,双江'

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'普洱熟茶中8氧化物咖啡因和嘧啶生物碱的存在_普洱茶知识_中华普洱茶网'

'晒青毛茶,双江勐库,临沧市,普洱茶,生茶,熟茶,勐库,临沧,绿茶,普洱,嘧啶,咖啡因,生物碱,层析,化合物,氧化,儿茶素,中分,吡喃糖,双江'

'普洱茶是以云南大叶茶[ Camellia sinensis(Linn .) var. assamica (Masters ) Kitamura ] 为原料制作的茶类。普洱茶可分为普洱生茶(Pu-Er raw tea) , 普洱陈茶( Pu-Er aging tea ) 和普洱熟茶(Pu-Er ripe tea) 等三...'

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  'field' => 
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array (
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    'pids' => '0',
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        'list' => 'list.html',
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        'list' => 'list.html',
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    'pids' => '0',
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      'linkurl' => '',
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        'list' => 'list.html',
        'search' => 'search.html',
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      'admin' => 
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      'urlrule' => 5,
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      'linkurl' => '',
      'seo' => 
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        'list' => 'list.html',
        'search' => 'search.html',
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      'admin' => 
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      'member' => 
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      'html' => 1,
      'urlrule' => 5,
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  30 => 
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    'pids' => '0',
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    'pdirname' => '',
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    'childids' => 30,
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    array (
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      'linkurl' => '',
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      'admin' => 
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      'admin' => 
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      'member' => 
      array (
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      'html' => 1,
      'urlrule' => 5,
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    'is_post' => 1,
    'tid' => 1,
    'url' => 'https://www.puerwang.cn/puerchajianfei/index.html',
    'total' => '请使用count标签查询',
    'field' => 
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  32 => 
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    'pids' => '0',
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    'pdirname' => '',
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    'disabled' => '0',
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    'childids' => 32,
    'thumb' => '',
    'show' => '1',
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      'linkurl' => '',
      'seo' => 
      array (
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        'show' => 'show.html',
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      'admin' => 
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      'member' => 
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      'urlrule' => 5,
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    'catids' => 
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    'is_post' => 1,
    'tid' => 1,
    'url' => 'https://www.puerwang.cn/puerchameirong/index.html',
    'total' => '请使用count标签查询',
    'field' => 
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  33 => 
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    'pids' => '0',
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      'linkurl' => '',
      'seo' => 
      array (
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        'category' => 'category.html',
        'list' => 'list.html',
        'search' => 'search.html',
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      'admin' => 
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          'add' => '1',
          'edit' => '1',
          'del' => '1',
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      'member' => 
      array (
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      'html' => 1,
      'urlrule' => 5,
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    'catids' => 
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    'is_post' => 1,
    'tid' => 1,
    'url' => 'https://www.puerwang.cn/chashangchanong/index.html',
    'total' => '请使用count标签查询',
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  34 => 
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    array (
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      'linkurl' => '',
      'seo' => 
      array (
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      'template' => 
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        'category' => 'category.html',
        'list' => 'list.html',
        'search' => 'search.html',
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      'admin' => 
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          'add' => '1',
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      'member' => 
      array (
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      'html' => 1,
      'urlrule' => 5,
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    'displayorder' => '0',
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    'catids' => 
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    'is_post' => 1,
    'tid' => 1,
    'url' => 'https://www.puerwang.cn/chajuchaji/index.html',
    'total' => '请使用count标签查询',
    'field' => 
    array (
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'/puerchazhishi/a-29121-[page].html'

''

'https://www.puerwang.cn/puerchazhishi/a-29121.html'

array (
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